P-212: The Effect of Ginger Hydro-Cohol Essence on Cyclophosphamide Side Effects in Spermatogenesis Processes

Background: Cyclophosphamide as an alkalizing and cytotoxic agent contributes in treatment of different kinds of cancer. It can pass the blood-brain barrier. Linking two strains of DNA in cell nucleus and breaking the inner and outer strain linkages and inhibiting RNA synthesis and as a consequence, inhibiting protein synthesis, cyclophosphamide demonstrates severe pharmacological side effects on the body. The most important cyclophosphamide side effects consist of azoospermia, reduction of gonadal hormones in hypothalamo-hypophyseal axis, reduction of sperm, infertility, cardio-vascular disorder, nephrotoxicity, bladder fibrosis and hemorrhagics cyct. Materials and Methods: In this study, forty adult male Balb/c mice (about 20 g) were examined in four groups of ten mice. A group was received neither solotion nor the drug, control group received only the vehicle and experimental groups received cyclophosphamide (5 mg/ Kg/BW) Intraperitoneally (IP). Then hydroalcoholic ginger extract was administered (1g/Kg/BW) orally along with cyclophosphamide in 14-day period. Results: In this study, Kruskal–Wallis test was used to analyse and calculate plasma concentrations of hypophyseal gonadotropic hormones (FSH, LH), testosterone and also spermatogenesis. Our result demonstrated that the experimental groups which received hydroalcoholic ginger extract simultaneously with the drug cyclophosphamide, significantly (p<0.05) had eleveted plasma concentrations of gonadotropic hormones and spermatogenesis related factors during receive of hydroalcoholic ginger extract. Conclusion: This work has shown that hydroalcoholic ginger extract (1g/Kg/BW) is able to reduce pharmacological side effects of cyclophosphamide (p<0.05).